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Proceedings Paper

3D in-vivo imaging of GFP-expressing T-cells in mice with non-contact fluorescence molecular tomography (Invited Paper)
Author(s): Anikitos Garofalakis; Heiko Meyer; Giannis Zacharakis; Eleftherios N. Economou; Clio Mamalaki; Joseph Papamatheakis; Vasilis Ntziachristos; Jorge Ripoll
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Paper Abstract

Optical imaging and tomography in tissues can facilitate the quantitative study of several important chromophores and fluorophores in-vivo. Due to this fact, there has been great interest in developing imaging systems offering quantitative information on the location and concentration of chromophores and fluorescent probes. In this study we present a novel imaging system that enables three dimensional (3D) imaging of fluorescent signals in bodies of arbitrary shapes in a non-contact geometry, in combination with a 3D surface reconstruction algorithm, which is appropriate for in-vivo small animal imaging of fluorescent probes. The system consists of a rotating sample holder and a lens coupled Charge Coupled Device (CCD) camera in combination with a fiber coupled laser scanning device. An Argon ion laser is used as the source and different filters are used for the detection of various fluorophores or fluorescing proteins. With this new setup a large measurements dataset can be achieved while the use of inversion models give a high capacity for quantitative 3D reconstruction of fluorochrome distributions as well as high spatial resolution. The system has already been tested in the observation of the distribution of Green Fluorescent Protein (GFP) expressing T-lymphocytes in order to study the function of the immune system in a murine model, which can then be related to the function of the human immune system.

Paper Details

Date Published: 22 June 2005
PDF: 10 pages
Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); doi: 10.1117/12.634830
Show Author Affiliations
Anikitos Garofalakis, Foundation for Research and Technology Hellas (Greece)
Heiko Meyer, Foundation for Research and Technology Hellas (Greece)
Giannis Zacharakis, Foundation for Research and Technology Hellas (Greece)
Eleftherios N. Economou, Foundation for Research and Technology Hellas (Greece)
Clio Mamalaki, Foundation for Research and Technology Hellas (Greece)
Joseph Papamatheakis, Foundation for Research and Technology Hellas (Greece)
Vasilis Ntziachristos, Massachusetts General Hospital, Harvard Medical School (United States)
Jorge Ripoll, Foundation for Research and Technology Hellas (Greece)


Published in SPIE Proceedings Vol. 5771:
Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI
Valery V. Tuchin, Editor(s)

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