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Proceedings Paper

The affect of low-coherent light on microbial colony forming ability and morphology of some gram-positive and gram-negative bacteria
Author(s): Denis Ed. Popov; Elena S. Tuchina; Julia A. Chernova; Dmitry Podshibyakin; Dmitry V. Rudik; Maria Samsonova; Igor Gromov; Valery V. Tuchin
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Paper Abstract

Gram-negative E. coli, gram-positive facultative anaerobe cocci Staphylococcus lugdensis, Micrococcus halobius, and Stomatococcus mucilaginosus as subjects of study were chosen. LEDs with spectrum maxima at 405 nm (without any exogenous sensitizer) and 660 nm (in conjunction with methylene blue) and power densities of 23 mW/cm2 and 5.7 mW/cm2 accordingly as continuous light sources were chosen. Photosensitized light's affect by methylene blue was studied on E. coli only. The original scheme of experiment set up was developed. It permits one to increase expositions quantity in each experiment for more certain trend's construction over dose curves and decrease parasite flora sowing. As a result of accomplished studies it was established that blue low-coherent light have unalike weak light's dose depending suppressing effect on cocci whereas red low-coherent light have a moderate dose-depended suppressing effect at low irradiation doses and a moderate dose-depended stimulating effect at high irradiation doses on sensitized by MeBlue E. coli. For all ofthis, but Staphylococcus morphology changes were observed.

Paper Details

Date Published: 22 June 2005
PDF: 4 pages
Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); doi: 10.1117/12.634773
Show Author Affiliations
Denis Ed. Popov, Saratov State Univ. (Russia)
Elena S. Tuchina, Saratov State Univ. (Russia)
Julia A. Chernova, Saratov State Univ. (Russia)
Dmitry Podshibyakin, Saratov State Univ. (Russia)
Dmitry V. Rudik, Saratov State Univ. (Russia)
Maria Samsonova, Saratov State Univ. (Russia)
Igor Gromov, Saratov State Univ. (Russia)
Valery V. Tuchin, Saratov State Univ. (Russia)

Published in SPIE Proceedings Vol. 5771:
Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI
Valery V. Tuchin, Editor(s)

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