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Proceedings Paper

Depth-resolved fluorescence of biological tissue
Author(s): Yicong Wu; Peng Xi; Tak-Hong Cheung; So Fan Yim; Mei-Yung Yu; Jianan Y. Qu
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Paper Abstract

The depth-resolved autofluorescence ofrabbit oral tissue, normal and dysplastic human ectocervical tissue within l20μm depth were investigated utilizing a confocal fluorescence spectroscopy with the excitations at 355nm and 457nm. From the topmost keratinizing layer of oral and ectocervical tissue, strong keratin fluorescence with the spectral characteristics similar to collagen was observed. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. Furthermore, NADH and FADfluorescence measured from the underlying non-keratinizing epithelial layer were strongly correlated to the tissue pathology. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.

Paper Details

Date Published: 22 June 2005
PDF: 4 pages
Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); doi: 10.1117/12.634626
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Peng Xi, Hong Kong Univ. of Science and Technology (Hong Kong China)
Tak-Hong Cheung, Chinese Univ. of Hong Kong, Prince of Wales Hospital (Hong Kong China)
So Fan Yim, Chinese Univ. of Hong Kong, Prince of Wales Hospital (Hong Kong China)
Mei-Yung Yu, Chinese Univ. of Hong Kong, Prince of Wales Hospital (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)


Published in SPIE Proceedings Vol. 5771:
Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI
Valery V. Tuchin, Editor(s)

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