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Proceedings Paper

Antimicrobial photodynamic treatment of gram-negative bacteria with a cationic phenothiazine dye under pulsed light irradiation
Author(s): Satoko Kawauchi; Shunichi Sato; Toru Yamaguchi; Nariyoshi Shinomiya; Daizo Saito; Hiroshi Ashida; Minoru Obara; Makoto Kikuchi
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Paper Abstract

In-vitro photodynamic inactivation of Ps. aeruginosa with methylene blue under pulsed light excitation was investigated at different pulse repetition rates. Bacterial suspensions were illuminated with 670-nm nanosecond pulsed light with a peak intensity of 2.0 MW/cm2 at pulse repetition rates in the range of 5-30 Hz. Photobactericidal effect increased with increasing pulse repetition rate for the same total light dose; more than two orders in magnitude reduction of bacterial survival fraction was obtained at 30 Hz. Such a positive dependence of photobactericidal effect on pulse repetition rate was inconsistent with our previous results for human lung cancer cells that were photodynamically treated with a lysosomal sensitizer. The reason for the increased photobactericidal effect at the high pulse repetition rate is discussed.

Paper Details

Date Published: 26 August 2005
PDF: 4 pages
Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630W (26 August 2005); doi: 10.1117/12.633093
Show Author Affiliations
Satoko Kawauchi, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Toru Yamaguchi, Keio Univ. (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Daizo Saito, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Minoru Obara, Keio Univ. (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)

Published in SPIE Proceedings Vol. 5863:
Therapeutic Laser Applications and Laser-Tissue Interactions II
Hubert van den Bergh; Alfred Vogel, Editor(s)

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