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Proceedings Paper

Femtosecond laser manipulation of subcellular organelles in living cells
Author(s): Wataru Watanabe; Tomoko Shimada; Kazuyoshi Itoh; Sachihiro Matsunaga; Kiichi Fukui
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Paper Abstract

Manipulation—that is, inactivation and movement—of subcellular organelles in living cells is important to understand cell dynamics. We review disruption of subcellular organelles in living cells by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of organelles in living cells was experimentally confirmed by restaining of organelles. Femtosecond laser based nanosurgery has the possibility to remove or manipulate organelles in living cells and may lead to elucidation of functional interactions between organelles, protein transportation, and inter-cell communication.

Paper Details

Date Published: 26 August 2005
PDF: 7 pages
Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630B (26 August 2005); doi: 10.1117/12.633079
Show Author Affiliations
Wataru Watanabe, Osaka Univ. (Japan)
Tomoko Shimada, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)
Sachihiro Matsunaga, Osaka Univ. (Japan)
Kiichi Fukui, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 5863:
Therapeutic Laser Applications and Laser-Tissue Interactions II
Hubert van den Bergh; Alfred Vogel, Editor(s)

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