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Proceedings Paper

Near-infrared Raman spectroscopy for colonic cancer diagnosis
Author(s): Zhiwei Huang; Wei Zheng; Sheppard Colin
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Paper Abstract

This study evaluated the diagnostic ability of near-infrared (NIR) Raman spectroscopy for identifying the malignant tumors from normal and benign tissues in the colon. In this work, a rapid NIR Raman system was utilized for tissue Raman studies. A total of 105 colonic specimens were used for Raman studies, including 41 normals, 18 polyps (benign), and 46 malignant tumors (22 of moderately differentiated adenocarcinomas and 24 of poorly differentiated adenocarcinomas). The results showed that high-quality Raman spectra in the 800-1800 cm-1 range can be acquired from human colonic tissues in vitro, and Raman spectra differed significantly between normal and malignant tumor tissue. The diagnostic algorithm using the Raman intensity ratios of I1085/1445 vs. I1002/1445 can yield a diagnostic sensitivity of 100% and specificity of 96.6% for differentiation between between normal, benign and malignant colonic tissue. This work demonstrates that NIR Raman spectroscopy technique has a significant potential for the noninvasive diagnosis of colon cancer in vivo based on the evaluation of changes of molecular vibrations of biomolecules in tissue.

Paper Details

Date Published: 7 October 2005
PDF: 5 pages
Proc. SPIE 5862, Diagnostic Optical Spectroscopy in Biomedicine III, 586204 (7 October 2005); doi: 10.1117/12.633006
Show Author Affiliations
Zhiwei Huang, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Sheppard Colin, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 5862:
Diagnostic Optical Spectroscopy in Biomedicine III
Mary-Ann Mycek, Editor(s)

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