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Proceedings Paper

Apoptosis induces temporal increase in attenuation as measured by optical coherence tomography
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Paper Abstract

Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis was induced. We analyzed the OCT data including both the scattering properties of the medium and the axial point spread function of the OCT system. We measured that the optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm-1 to 1.3 ± 0.6 mm-1, whereas with the apoptotic cells a clear increase (up to 6.4 ± 1.7 mm-1) in scattering was observed. The results on cultured cells a presented in this study indicate the ability of OCT to detect and differentiate between viable, apoptotic and necrotic cells based on their backscatter properties. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on line monitoring of tissues, e.g. for feedback in cancer treatment.

Paper Details

Date Published: 7 October 2005
PDF: 10 pages
Proc. SPIE 5861, Optical Coherence Tomography and Coherence Techniques II, 58610Y (7 October 2005); doi: 10.1117/12.632992
Show Author Affiliations
Freek J van der Meer, Laser Ctr., Academic Medical Ctr., Univ. of Amsterdam (Netherlands)
Interuniversity Cardiology Institute of the Netherlands (Netherlands)
Dirk J. Faber, Laser Ctr., Academic Medical Ctr., Univ. of Amsterdam (Netherlands)
Maurice C.G. Aalders, Laser Ctr., Academic Medical Ctr., Univ. of Amsterdam (Netherlands)
Anthonie van Leeuwenhoek Hospital (Netherlands)
Jop Perree, Laser Ctr., Academic Medical Ctr., Univ. of Amsterdam (Netherlands)
Ton G. van Leeuwen, Laser Ctr., Academic Medical Ctr., Univ. of Amsterdam (Netherlands)
Univ. of Twente (Netherlands)


Published in SPIE Proceedings Vol. 5861:
Optical Coherence Tomography and Coherence Techniques II
Wolfgang Drexler, Editor(s)

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