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Proceedings Paper

Multifocal multiphoton fluorescence lifetime microsocpy for biomedical applications
Author(s): A. Deniset; S. Leveque-Fort; M. P. Fontaine-Aupart; G. Roger; P. Georges
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Paper Abstract

Two-photon microscopy is a key method for biological and medical research on cells and tissues mainly due to the submicronic spatial resolution. Unfortunately in its conventional form, this technique leads to long time recording for three-dimensional and fluorescence lifetime imaging because it requires a single point laser scanning. The most suitable way to improve acquisition time is to illuminate the biological sample with several excitation points simultaneously. We thus present a time-resolved multifocal multiphoton microscope. Besides the advantage of preserving biological samples by reducing by a factor 64 the exposition time, this method keeps also the possibility of measuring both intensity and lifetime images of the samples.

Paper Details

Date Published: 7 October 2005
PDF: 8 pages
Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600J (7 October 2005); doi: 10.1117/12.632951
Show Author Affiliations
A. Deniset, Lab. de Photophysique Moleculaire, CNRS, Univ. Paris-Sud (France)
S. Leveque-Fort, Lab. de Photophysique Moleculaire, CNRS, Univ. Paris-Sud (France)
M. P. Fontaine-Aupart, Lab. de Photophysique Moleculaire, CNRS, Univ. Paris-Sud (France)
G. Roger, Lab. Charles Fabry, Institut d'Optique, CNRS, Univ. Paris-Sud (France)
P. Georges, Lab. Charles Fabry, Institut d'Optique, CNRS, Univ. Paris-Sud (France)


Published in SPIE Proceedings Vol. 5860:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging II
Tony Wilson, Editor(s)

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