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Proceedings Paper

Multiphoton imaging of an in vitro ovarian tissue model
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Paper Abstract

In order to understand the distribution of endogenous fluorescence in the ovary, ovarian biopsies were maintained with a viable tissue imaging system and characterized with multiphoton imaging. It was imperative to maintain a stable in vitro environment so that tissue images could provide accurate correlative data for in vivo spectroscopic measurements. Evaluating tissue viability in real time poses a difficult task given that viability assays are tailored for cell culture. The focus of this study was to design a robust in vitro imaging chamber for assessment of ovarian autofluorescence and simple, reliable viability assays for tissue status monitoring.

Paper Details

Date Published: 7 October 2005
PDF: 8 pages
Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 586002 (7 October 2005); doi: 10.1117/12.632938
Show Author Affiliations
Nathaniel D. Kirkpatrick, Univ. of Arizona (United States)
Molly A Brewer, Univ. of Arizona (United States)
Urs Utzinger, Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 5860:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging II
Tony Wilson, Editor(s)

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