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Proceedings Paper

Digital holographic microscopy for study cellular dynamics
Author(s): Pierre Marquet; Benjamin Rappaz; Etienne Cuche; Yves Emery; Christian D. Depeursinge; Pierre Magistretti
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Paper Abstract

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DHM images of living cells in culture are presented. They represent the distribution of the optical path length over the cell, which contains information concerning both the cellular morphometry and the intracellular refractive index, and which has been measured with a sub-wavelength accuracy.

Paper Details

Date Published: 1 September 2005
PDF: 4 pages
Proc. SPIE 5864, Novel Optical Instrumentation for Biomedical Applications II, 58640C (1 September 2005); doi: 10.1117/12.632917
Show Author Affiliations
Pierre Marquet, Lausanne Univ. (Switzerland)
Benjamin Rappaz, Lausanne Univ. (Switzerland)
Etienne Cuche, Lyncee Tec SA (Switzerland)
Yves Emery, Lyncee Tec SA (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Federale de Lausanne (Switzerland)
Pierre Magistretti, Lausanne Univ. (Switzerland)

Published in SPIE Proceedings Vol. 5864:
Novel Optical Instrumentation for Biomedical Applications II
Christian D. Depeursinge, Editor(s)

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