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Proceedings Paper

Time-resolved fluorescence measurements using stroboscopic excitation
Author(s): Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Rachel J. Errington; Paul J. Smith; Simon Ameer-Beg; Boris Vojnovic
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Paper Abstract

We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip". A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.

Paper Details

Date Published: 1 September 2005
PDF: 7 pages
Proc. SPIE 5864, Novel Optical Instrumentation for Biomedical Applications II, 586409 (1 September 2005); doi: 10.1117/12.632914
Show Author Affiliations
Daniel R. Matthews, Cardiff Univ. (United Kingdom)
Huw D. Summers, Cardiff Univ. (United Kingdom)
Kerenza Njoh, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Paul J. Smith, Cardiff Univ. (United Kingdom)
Simon Ameer-Beg, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Boris Vojnovic, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)


Published in SPIE Proceedings Vol. 5864:
Novel Optical Instrumentation for Biomedical Applications II
Christian D. Depeursinge, Editor(s)

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