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Proceedings Paper

Sensing cellular function and molecular activity in vivo using fluorescence lifetime imaging microscopy (FLIM)
Author(s): Wei Zhong; Dhruv Sud; Mei Wu; Karl A. Merrick; Sofia D. Merajver; David G. Beer; Mary-Ann Mycek
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Paper Abstract

In contrast to intensity-based fluorescence microscopy, fluorescence lifetime imaging microscopy (FLIM) bases image contrast on fluorophore excited-state lifetime. This technique is sensitive to the fluorophore's local environment (temperature, ion concentration, dissolved gas concentration, and molecular associations), while being independent of factors impacting fluorescence intensity (fluorophore concentration, photobleaching, scattering, and absorption). We present design features of a novel UV-visible-NIR wide-field time-domain FLIM system with optical sectioning (10 μm), high temporal discrimination (50 ps), and large temporal dynamic range (750 ps - 1 μs), and apply the system to probe cellular metabolic function and detect molecular activity in vivo.

Paper Details

Date Published: 1 September 2005
PDF: 9 pages
Proc. SPIE 5864, Novel Optical Instrumentation for Biomedical Applications II, 586407 (1 September 2005); doi: 10.1117/12.632912
Show Author Affiliations
Wei Zhong, Univ. of Michigan (United States)
Dhruv Sud, Univ. of Michigan (United States)
Mei Wu, Univ. of Michigan Medical School (United States)
Karl A. Merrick, Univ. of Michigan Medical School (United States)
Sofia D. Merajver, Univ. of Michigan Medical School (United States)
David G. Beer, Univ. of Michigan Medical School (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)
Univ. of Michigan Medical School (United States)


Published in SPIE Proceedings Vol. 5864:
Novel Optical Instrumentation for Biomedical Applications II
Christian D. Depeursinge, Editor(s)

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