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Proceedings Paper

Frequency-domain optical diffusion tomography of fluorescent proteins
Author(s): Ilya V. Turchin; Vladimir I. Plehanov; Ekaterina A. Sergeeva; Anna G. Orlova; Vladimir A. Vorob'ev; Vladislav A. Kamensky; Alexander P. Savitsky
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Paper Abstract

We present preliminary results of the frequency domain fluorescent diffuse tomography (FD FDT) method in application to fluorescent proteins. For first step in the experimental setup we utilized light-emitting diode (530 nm wavelength) modulated with low frequency (18 kHz). A model experiments with capsules containing DsRed suspension in scattering medium has been conducted. The results of post mortem experiments with capsules containing DsRed, introduced into abdominal cavity of mice to simulate tumors inside animal body, are presented. An algorithm of processing fluorescent image based on calculating zero of maximum curvature has been applied to detect fluorescent inclusions boundaries on the image.

Paper Details

Date Published: 8 October 2005
PDF: 6 pages
Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 58591H (8 October 2005); doi: 10.1117/12.632879
Show Author Affiliations
Ilya V. Turchin, Institute of Applied Physics, RAS (Russia)
Vladimir I. Plehanov, Institute of Applied Physics, RAS (Russia)
Ekaterina A. Sergeeva, Institute of Applied Physics, RAS (Russia)
Anna G. Orlova, Institute of Applied Physics, RAS (Russia)
Vladimir A. Vorob'ev, Institute of Applied Physics, RAS (Russia)
Vladislav A. Kamensky, Institute of Applied Physics, RAS (Russia)
Alexander P. Savitsky, A.N. Bach Institute of Biochemistry, RAS (Russia)


Published in SPIE Proceedings Vol. 5859:
Photon Migration and Diffuse-Light Imaging II
Kai Licha; Rinaldo Cubeddu, Editor(s)

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