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Proceedings Paper

Interferometric tracking of two particles with dynamic optical traps
Author(s): Dirk Neumayer; Peter Kaiser; Ernst H.K. Stelzer; Alexander Rohrbach
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Paper Abstract

Optical potential landscapes for diffusing particles can be generated by time-multiplexing (scanning) an optical trap and additionally modulating the trapping laser power. We show that it is possible to determine the lateral and axial positions of several particles in parallel with some 10 nm precision and at kHz rates by using dynamic back focal plane interferometry. This allows measuring the interaction of diffusing, (non-) functionalized particles in a confined volume. The scan frequency of the optical trap can be optimized for interaction measurements with high dynamic or temporal resolution, given a data sampling frequency of 1MHz.

Paper Details

Date Published: 7 October 2005
PDF: 7 pages
Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 58590A (7 October 2005); doi: 10.1117/12.632831
Show Author Affiliations
Dirk Neumayer, European Molecular Biology Lab. (Germany)
Peter Kaiser, European Molecular Biology Lab. (Germany)
Ernst H.K. Stelzer, European Molecular Biology Lab. (Germany)
Alexander Rohrbach, European Molecular Biology Lab. (Germany)

Published in SPIE Proceedings Vol. 5859:
Photon Migration and Diffuse-Light Imaging II
Kai Licha; Rinaldo Cubeddu, Editor(s)

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