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Proceedings Paper

Measurement of intracellular oxygen levels using fluorescence lifetime imaging microscopy (FLIM)
Author(s): Dhruv Sud; Wei Zhong; David Beer; Mary-Ann Mycek
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Paper Abstract

Intracellular oxygen levels were measured in vivo under physiological-temperature controlled conditions by monitoring the fluorescence lifetime of the oxygen sensitive dye ruthenium tris(2,2'-dipyridyl) dichloride hexahydrate (RTDP). We employed fluorescence lifetime imaging microscopy (FLIM) and an independent oxygen sensor to calibrate changes in RTDP lifetime with corresponding changes in oxygen level. The FLIM method reproducibly quantified oxygen levels in living human cells cultured from a (Barrett's) adenocarcinoma columnar cell line (SEG-1). Approaches such as those developed here should prove useful for studying oxygen gradients in 3-D biological specimens and for monitoring cellular metabolic status.

Paper Details

Date Published: 7 October 2005
PDF: 10 pages
Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 585907 (7 October 2005); doi: 10.1117/12.632827
Show Author Affiliations
Dhruv Sud, Univ. of Michigan (United States)
Wei Zhong, Univ. of Michigan (United States)
David Beer, Univ. of Michigan Medical Ctr. (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)
Univ. of Michigan Medical School (United States)

Published in SPIE Proceedings Vol. 5859:
Photon Migration and Diffuse-Light Imaging II
Kai Licha; Rinaldo Cubeddu, Editor(s)

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