Share Email Print
cover

Proceedings Paper

Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object.

Paper Details

Date Published: 7 October 2005
PDF: 6 pages
Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 585906 (7 October 2005); doi: 10.1117/12.632826
Show Author Affiliations
Jenny Svensson, Lund Univ. Medical Laser Ctr., Lund Institute of Technology (Sweden)
Anikitos Garofalakis, Institute of Electronic Structure and Laser-FORTH (Greece)
Heiko Meyer, Institute of Electronic Structure and Laser-FORTH (Greece)
Florian Forster, Lund Univ. Medical Laser Ctr., Lund Institute of Technology (Sweden)
Jorge Ripoll, Institute of Electronic Structure and Laser-FORTH (Greece)
Stefan Andersson-Engels, Lund Univ. Medical Laser Ctr., Lund Institute of Technology (Sweden)


Published in SPIE Proceedings Vol. 5859:
Photon Migration and Diffuse-Light Imaging II
Kai Licha; Rinaldo Cubeddu, Editor(s)

© SPIE. Terms of Use
Back to Top