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Proceedings Paper

Flow through PCR module of BioBriefcase
Author(s): E. Arroyo; E. K. Wheeler; R. Shediac; B. Hindson; S. Nasarabadi; G. Vrankovich; P. Bell; C. Bailey; T. Sheppod; A. T. Christian
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Paper Abstract

The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed with a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.

Paper Details

Date Published: 11 November 2005
PDF: 9 pages
Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600714 (11 November 2005); doi: 10.1117/12.630844
Show Author Affiliations
E. Arroyo, Lawrence Livermore National Lab. (United States)
E. K. Wheeler, Lawrence Livermore National Lab. (United States)
R. Shediac, Sandia National Lab. (United States)
B. Hindson, Lawrence Livermore National Lab. (United States)
S. Nasarabadi, Lawrence Livermore National Lab. (United States)
G. Vrankovich, Lawrence Livermore National Lab. (United States)
P. Bell, Lawrence Livermore National Lab. (United States)
C. Bailey, Lawrence Livermore National Lab. (United States)
T. Sheppod, Sandia National Lab. (United States)
A. T. Christian, Lawrence Livermore National Lab. (United States)


Published in SPIE Proceedings Vol. 6007:
Smart Medical and Biomedical Sensor Technology III
Brian M. Cullum; J. Chance Carter, Editor(s)

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