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Proceedings Paper

A portable cell-based optical detection device for rapid detection of Listeria and Bacillus toxins
Author(s): Pratik Banerjee; Padmapriya P. Banada; Jenna L. Rickus; Mark T. Morgan; Arun K. Bhunia
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Paper Abstract

A mammalian cell-based optical biosensor was built to detect pathogenic Listeria and Bacillus species. This sensor measures the ability of the pathogens to infect and induce cytotoxicity on hybrid lymphocyte cell line (Ped-2E9) resulting in the release of alkaline phosphatase (ALP) that can be detected optically using a portable spectrophotometer. The Ped-2E9 cells were encapsulated in collagen gel matrices and grown in 48-well plates or in specially designed filtration tube units. Toxin preparations or bacterial cells were introduced and ALP release was assayed after 3-5 h. Pathogenic L. monocytogenes strains or the listeriolysin toxins preparation showed cytotoxicity ranging from 55% - 92%. Toxin preparations (~20 μg/ml) from B. cereus strains showed 24 - 98% cytotoxicity. In contrast, a non-pathogenic L. innocua (F4247) and a B. substilis induced only 2% and 8% cytotoxicity, respectively. This cell-based detection device demonstrates its ability to detect the presence of pathogenic Listeria and Bacillus species and can potentially be used onsite for food safety or in biosecurity application.

Paper Details

Date Published: 8 November 2005
PDF: 7 pages
Proc. SPIE 5996, Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality, 599602 (8 November 2005); doi: 10.1117/12.630793
Show Author Affiliations
Pratik Banerjee, Purdue Univ. (United States)
Padmapriya P. Banada, Purdue Univ. (United States)
Jenna L. Rickus, Purdue Univ. (United States)
Mark T. Morgan, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 5996:
Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality
Yud-Ren Chen; George E. Meyer; Shu-I Tu, Editor(s)

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