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Proceedings Paper

Electrochemical biosensors for rapid determination of pesticide residues in agricultural products
Author(s): Xuesong Jiang; Jianping Wang; Yibin Ying; Zunzhong Ye; Yanbin Li
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Paper Abstract

The biosensors, consisting of immobilized antibodies which were for specific recognition to target molecules and electrodes which were able to convert the binding event between antigen and antibody to a detectable signal, were developed for rapid detection of organophosphate (OPs) pesticides. Anti-OPs antibodies were immobilized onto indium-tin-oxide (ITO) coated interdigitated microsensor electrodes (IMEs). The Faradaic impedance spectra, presented as Nyquist plots (Z' vs Z'') and Bode diagrams, (impedance vs frequency) were recorded in the frequency range from 1Hz to 100 kHz respectively. A linear relationship between the electron-transfer resistance and concentrations of OPs pesticide was found ranging from 0.1 ppm to 100 ppm. The regression equations were Y = 658 X +1861, with the correlation coefficient of 0.977. The biosensing procedure was simple and rapid, and could be completed within 1 h.

Paper Details

Date Published: 9 November 2005
PDF: 10 pages
Proc. SPIE 5994, Chemical and Biological Sensors for Industrial and Environmental Security, 59940U (9 November 2005); doi: 10.1117/12.630596
Show Author Affiliations
Xuesong Jiang, Zhejiang Univ. (China)
Jianping Wang, Zhejiang Univ. (China)
Yibin Ying, Zhejiang Univ. (China)
Zunzhong Ye, Zhejiang Univ. (China)
Yanbin Li, Zhejiang Univ. (China)


Published in SPIE Proceedings Vol. 5994:
Chemical and Biological Sensors for Industrial and Environmental Security
Arthur J. Sedlacek; Steven D. Christesen; Roger J. Combs; Tuan Vo-Dinh, Editor(s)

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