Share Email Print
cover

Proceedings Paper

Enhancement of biomolecular detection sensitivity by surface plasmon resonance ellipsometry
Author(s): Hyun Mo Cho; Won Chegal; Yong Jai Cho; Young-pil Kim; Hak-sung Kim
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We present the application of ellipsometry to the phase measurement of surface plasmon resonance (SPR) in biomolecular detection. In this configuration, the phase measurement gives a large enhancement of detection sensitivity in comparison to traditional SPR techniques. In this work, the experimental setup for SPR ellipsometry is based on both custom-built rotating analyzer ellipsometer and an imaging ellipsometer which are equipped with a SPR-cell and a flow system, respectively. We investigate the adequate thickness of the gold layer used for SPR cell and the resolution of the phase detection using two ellipsometric methods under the SPR condition. The rotating analyzer method yields higher sensitivity sufficient to detect changes in the effective thickness of biomolecular layers of less than 1 pm. In comparison to conventional SPR the simultaneous measurement of ellipsometric parameters, Δ and ψ, yields more information which is useful for quantitative analysis based on fitting theoretical solutions to experimental results.

Paper Details

Date Published: 17 November 2005
PDF: 6 pages
Proc. SPIE 6008, Nanosensing: Materials and Devices II, 60081F (17 November 2005); doi: 10.1117/12.630379
Show Author Affiliations
Hyun Mo Cho, Korea Research Institute of Standards and Science (South Korea)
Won Chegal, Korea Research Institute of Standards and Science (South Korea)
Yong Jai Cho, Korea Research Institute of Standards and Science (South Korea)
Young-pil Kim, Korea Advanced Institute of Science and Technology (South Korea)
Hak-sung Kim, Korea Advanced Institute of Science and Technology (South Korea)


Published in SPIE Proceedings Vol. 6008:
Nanosensing: Materials and Devices II
M. Saif Islam; Achyut K. Dutta, Editor(s)

© SPIE. Terms of Use
Back to Top