Share Email Print
cover

Proceedings Paper

Synthesis and characterization of peptide nanostructures designed for sensing applications
Author(s): Normand Voyer; Mathieu Arseneault; Francois Otis
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We report the design and the synthesis of membrane-active peptide nanostructures, as well as their use as signal transducer in a fluorimetric assay for biologically relevant analytes. Addition of hydrophobic 21-residue peptides bearing six crown ether side chains to a solution of small unilamellar vesicles loaded with a pH-sensitive fluorophore induces a rapid fluorescence increase associated with Na+/H+ transport across the bilayer membrane. To demonstrate the usefulness of these peptide nanostructures in the development of simple, rapid, and sensitive detection assays for a wide range of analytes, peptide nanostructures bearing a biotin at the N-terminal position were prepared. Addition of avidin to the assay employing these modified peptides resulted in a significant change in the time-dependent fluorescence profile. Control experiments with a non-binding proteins and saturated avidin showed that the observed changes are indeed due to specific binding of avidin to biotin modified nanostructures.

Paper Details

Date Published: 12 October 2005
PDF: 8 pages
Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59690M (12 October 2005); doi: 10.1117/12.628791
Show Author Affiliations
Normand Voyer, Univ. Laval (Canada)
Mathieu Arseneault, Univ. Laval (Canada)
Francois Otis, Univ. Laval (Canada)


Published in SPIE Proceedings Vol. 5969:
Photonic Applications in Biosensing and Imaging
Brian C. Wilson; Richard I. Hornsey; Warren C. W. Chan; Ulrich J. Krull; Robert A. Weersink; Kui Yu, Editor(s)

© SPIE. Terms of Use
Back to Top