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Proceedings Paper

Multi-modal miniature microscope - 4M device for bio-imaging applications: an overview of the system
Author(s): Tomasz S. Tkaczyk; Jeremy D. Rogers; Mohammed Rahman; Todd C. Christenson; Stephen Gaalema; Eustace L. Dereniak; Rebecca Richards-Kortum; Michael R. Descour
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Paper Abstract

The multi-modal miniature microscope (4M) device to image morphology and cytochemistry in vivo is a microscope on a chip including optical, micro-mechanical, and electronic components. This paper describes all major system components: optical system, custom high speed CMOS detector and comb drive actuator. The hybrid sol-gel lenses, their fabrication and assembling technology, optical system parameters, and various operation modes (fluorescence, reflectance, structured illumination) are also discussed. A particularly interesting method is a structured illumination technique that delivers confocal-imaging capabilities and may be used for optical sectioning. For reconstruction of the sectioned layer a sine approximation algorithm is applied. Structured illumination is produced with LIGA fabricated actuator scanning in resonance. The spatial resolution of the system is 1 μm, and was magnified by 4x matching the CMOS pixel size of 4 μm (a lateral magnification is 4:1), and the extent of field of the system is 250μm. An overview of the 4M device is combined with the presentation of imaging results for epithelial cell phantoms with optical properties characteristic of normal and cancerous tissue labeled with nanoparticles.

Paper Details

Date Published: 24 September 2005
PDF: 9 pages
Proc. SPIE 5959, Medical Imaging, 59590N (24 September 2005); doi: 10.1117/12.626169
Show Author Affiliations
Tomasz S. Tkaczyk, College of Optical Sciences, Univ. of Arizona (United States)
Jeremy D. Rogers, College of Optical Sciences, Univ. of Arizona (United States)
Mohammed Rahman, Univ. of Texas (United States)
Todd C. Christenson, HT Micro (United States)
Stephen Gaalema, Black Forest Engineering (United States)
Eustace L. Dereniak, College of Optical Sciences, Univ. of Arizona (United States)
Rebecca Richards-Kortum, Univ. of Texas (United States)
Michael R. Descour, College of Optical Sciences, Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 5959:
Medical Imaging
Andrzej Kowalczyk; Adolf F. Fercher; Valery V. Tuchin, Editor(s)

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