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Proceedings Paper

FRET based biosensor for detection of active NF-kB
Author(s): Francesco Baldini; Lorenzo Citti; Claudio Domenici; Ambra Giannetti; Lorena Tedeschi; Tuan Vo-Dinh; Musundi B. Wabuyele
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Paper Abstract

The Nuclear Factor kB is a transcription factor, ubiquitously expressed, involved in the regulation of a large number of genes and in a variety of human disease including inflammation, asthma, atherosclerosis, AIDS, septic shock, arthritis and cancer. The critical need for a simple and direct method to evaluate the quantity of active NF-kB in a biological sample can be addressed using a suitable and reusable biosensor. For this purpose, a novel method, using fluorescence resonance energy transfer (FRET), to detect the active form of NF-kB binding a specific DNA sequence has been developed. A single-stranded DNA (ssDNA) with auto-complementary sequence has been properly designed and synthesized. In order to evaluate FRET due to the DNA/protein binding interaction taking place between double-stranded DNA (dsDNA) immobilized in a capillary wall and NF-kB proteins, a highly sensitive FRET-based biosensor system developed in our laboratory was used. Preliminary results show that our system was capable of detecting the active form of NF-kB protein with a detection efficiency of about 90% and that the system has a good regenerability.

Paper Details

Date Published: 23 May 2005
PDF: 4 pages
Proc. SPIE 5855, 17th International Conference on Optical Fibre Sensors, (23 May 2005); doi: 10.1117/12.623696
Show Author Affiliations
Francesco Baldini, Institute for Applied Physics, CNR (Italy)
Lorenzo Citti, Institute of Clinical Physiology, CNR (Italy)
Claudio Domenici, Institute of Clinical Physiology, CNR (Italy)
Ambra Giannetti, Institute for Applied Physics, CNR (Italy)
Lorena Tedeschi, Institute of Clinical Physiology, CNR (Italy)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)
Musundi B. Wabuyele, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 5855:
17th International Conference on Optical Fibre Sensors
Marc Voet; Reinhardt Willsch; Wolfgang Ecke; Julian Jones; Brian Culshaw, Editor(s)

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