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Proceedings Paper

Optical fibre biosensors for oxygen and glucose monitoring
Author(s): J. S. Young; Patricia J. Scully; F. Kvasnik; K. Rose; Gabriela Kuncova; O. Podrazky; Vlastimil Matejec; Jan Mrazek
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Paper Abstract

An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER) is described. The Ruthenium/ ORMOCER layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

Paper Details

Date Published: 23 May 2005
PDF: 4 pages
Proc. SPIE 5855, 17th International Conference on Optical Fibre Sensors, (23 May 2005); doi: 10.1117/12.623688
Show Author Affiliations
J. S. Young, The Univ. of Manchester (United Kingdom)
Patricia J. Scully, The Univ. of Manchester (United Kingdom)
F. Kvasnik, Laser Technology North West Ltd. (United Kingdom)
K. Rose, Fraunhofer-Institut fuer Silicatforschung (Germany)
Gabriela Kuncova, Institute of Chemical Process Fundamentals (Czech Republic)
O. Podrazky, Institute of Chemical Process Fundamentals (Czech Republic)
Vlastimil Matejec, Institute of Radio Engineering and Electronics (Czech Republic)
Jan Mrazek, Institute of Radio Engineering and Electronics (Czech Republic)


Published in SPIE Proceedings Vol. 5855:
17th International Conference on Optical Fibre Sensors
Marc Voet; Reinhardt Willsch; Wolfgang Ecke; Julian Jones; Brian Culshaw, Editor(s)

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