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Proceedings Paper

Finite element modeling of the micropipette aspiration of malaria-infected red blood cells
Author(s): E. H. Zhou; C. T. Lim; K. S. W. Tan; Ser Tong Quek
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Paper Abstract

Micropipette aspiration is one of the most widely used techniques for measuring the mechanical properties of single cells. The homogeneous linear elastic half-space model has been frequently applied to characterize the micropipette aspiration of chondrocytes and endothelial cells. However, the linear elastic model is limited to small deformation and the half-space assumption is frequently invalidated when moderately large micropipettes are used. In this work, the linear elastic constitutive model is extended to the neo-Hookean constitutive model and the geometry is simulated more realistically by considering the cell as a sphere. The large-deformation contact mechanics problem is solved using dimensionless axisymmetric finite element analysis. The effects of pipette diameter and fillet radius on the cellular rheological behaviour are also systematically studied. Based on the finite element simulation, empirical relationships have been derived for the direct interpretation of the elastic mechanical parameters from the micropipette aspiration experiments. Micropipette aspiration of late-stage malaria-infected red blood cells (schizonts) is conducted. The infected cells are found to exhibit elastic solid behavior in contrast to the liquid drop behavior of healthy red blood cells. The apparent shear modulus of the schizonts, interpreted from the elastic solid model, is found to be 119±62 Pa.

Paper Details

Date Published: 12 April 2005
PDF: 5 pages
Proc. SPIE 5852, Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, (12 April 2005); doi: 10.1117/12.621911
Show Author Affiliations
E. H. Zhou, National Univ. of Singapore (Singapore)
C. T. Lim, National Univ. of Singapore (Singapore)
K. S. W. Tan, National Univ. of Singapore (Singapore)
Ser Tong Quek, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 5852:
Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics
Chenggen Quan; Fook Siong Chau; Anand Asundi; Brian Stephen Wong; Chwee Teck Lim, Editor(s)

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