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Proceedings Paper

Optics-based sensor for DNA screening applications
Author(s): Wei Ming Chiang; Chu Sing Lim; Tet Fatt Chia; Wai Fun Tan; Seng Kim Lim
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Paper Abstract

Short Tandem Repeats (STRs) are non-coding regions in genomic DNA that consist of short segments of core tandem repeat sequences. As these sequences are highly polymorphic in nature, they are extremely specific and unique to the individual. The STR fragments are amplified through the Polymerase Chain Reaction (PCR) before they are separated and analyzed. In this study, an optic-based system was developed to detect two STR loci in a gel matrix. Results show that the system is able to detect the STR fragments with a precision of +/- 1.0 bp, which is considered acceptable, given that the chosen STR loci have 4 bp repeat sequences. This system offers an inexpensive yet efficient method of STR DNA typing.

Paper Details

Date Published: 12 April 2005
PDF: 7 pages
Proc. SPIE 5852, Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, (12 April 2005); doi: 10.1117/12.621180
Show Author Affiliations
Wei Ming Chiang, Nanyang Technological Univ. (Singapore)
Chu Sing Lim, Nanyang Technological Univ. (Singapore)
Tet Fatt Chia, Nanyang Technological Univ. (Singapore)
Wai Fun Tan, Health Sciences Authority (Singapore)
Seng Kim Lim, Singapore Police Force (Singapore)


Published in SPIE Proceedings Vol. 5852:
Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics

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