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Proceedings Paper

Variation of entropic elasticity of DNA-Psoralen complex under UV light
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Paper Abstract

We measure the entropic elasticity of a single λ-DNA molecule and of a single DNA-Psoralen complex by doing stretching experiments with an optical tweezers. Psoralen is a photosensitive drug used in the treatment of many skin diseases, by impeding DNA replication. Psolaren intercalates the DNA and can form crosslinks with pyrimidine basis in opposite strands of DNA, when illuminated with UVA light. As crosslinks form the persistence length of the complex increases, indicating an increase in rigidity of the complex. We study the kinetics of DNA-Psoralen crosslink formation via changes in entropic elasticity of the complex.

Paper Details

Date Published: 26 August 2005
PDF: 9 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593011 (26 August 2005); doi: 10.1117/12.619128
Show Author Affiliations
M. S. Rocha, Univ. Federal de Minas Gerais (Brazil)
O. N. Mesquita, Univ. Federal de Minas Gerais (Brazil)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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