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Proceedings Paper

In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells
Author(s): Radostina Alexandrova; Elena Stoykova; Rodica-Mariana Ion; Kristina Nedkova; Elena Ivanova; Kaloian Zdravkov; Georgi Minchev
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Paper Abstract

In this study we evaluate in vitro the cytotoxic activity of two newly synthesized phthalocyanines (metallo-complexes) with absorption bands in the red part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS4Pc). Two permanent animal tumor cell lines are used in the experiments: LSR-SF(SR)-transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and LSCC-SF(Mc29)-transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29. Irradiation has been performed with a laser diode emitting at 672 nm in a wide range of radiant exposure (2-100 J/cm2) and irradiance of 120 mW/cm2. The neutral red uptake cytotoxicity assay has been used to evaluate the effect of the photosensitizers on cell viability. The light dose-response curves and light exposures that ensure viability drop to 50% and 10% have been obtained for each cell line. The ability of the compounds to induce apoptosis has been determined by acridine orange dye staining.

Paper Details

Date Published: 22 April 2005
PDF: 5 pages
Proc. SPIE 5830, 13th International School on Quantum Electronics: Laser Physics and Applications, (22 April 2005); doi: 10.1117/12.618977
Show Author Affiliations
Radostina Alexandrova, Institute of Experimental Pathology and Parasitology (Bulgaria)
Elena Stoykova, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Rodica-Mariana Ion, Institute for Chemical Research (Romania)
Kristina Nedkova, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Elena Ivanova, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Kaloian Zdravkov, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Georgi Minchev, Central Lab. of Optical Storage and Processing of Information (Bulgaria)


Published in SPIE Proceedings Vol. 5830:
13th International School on Quantum Electronics: Laser Physics and Applications
Peter A. Atanasov; Sanka V. Gateva; Lachezar A. Avramov; Alexander A. Serafetinides, Editor(s)

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