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Proceedings Paper

Monitoring sperm mitochondrial respiration response in a laser trap using ratiometric fluorescence
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Paper Abstract

Sperm motility is an important area in understanding male infertility. Various techniques, such as the Computer Assisted Sperm Analysis (CASA), have been used to understand sperm motility. Sperm motility is related to the energy (ATP) production of sperm. ATP is produced by the depolarization of the membrane potential of the inner membrane of the mitochondria. In this study, a mitochondrial dye, JC-1, has been used to monitor the energetics of the mitochondria. This fluorescent dye can emit at two different wavelengths, depending on the membrane potential of the mitochondria. It can fluoresce green at low membrane potential and red at high membrane potential. The ratio of the two colors (red/green) allows for an accurate measurement of the change of membrane potential. Various experiments were conducted to quantify the behavior of the dye within the sperm and the reaction of the sperm to trap. Sperm were trapped using laser tweezers. Results have shown that the ratio drops dramatically when sperm are trapped, indicating a depolarization of the membrane. The physiological response to this depolarization is yet to be determined, but the studies indicate that the sperm could have been slightly damaged by the laser. However, knowing that sperm depolarizes their membrane when trapped can help understand how sperm react to their environment and consequently help treat male infertility.

Paper Details

Date Published: 18 August 2005
PDF: 11 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59302F (18 August 2005); doi: 10.1117/12.618126
Show Author Affiliations
Adrian Mei, Univ. of California, San Diego (United States)
Elliot Botvinick, Beckman Laser Institute and Medical Clinic (United States)
Michael Berns, Beckman Laser Institute and Medical Clinic (United States)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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