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Proceedings Paper

Coherent imaging by digital holographic microscopy: focusing capabilities and depth of focus in the reconstructed images
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Paper Abstract

Observing at a microscope object having a three-dimensional (3D) complex shape only a portion of it appears in focus since essentially a single image plane is imaged. Up to now only two approaches have been developed to obtain an extended focused image (EFI). An EFI shows all details of the object in focus. Both methods having severe limitations since one requires mechanical scanning while the other needs specially designed optics. We demonstrate that an EFI image of an object can be obtained by means of digital holography (DH) in a microscope configuration overcoming the mentioned limitations. The novelty of the proposed approach lies in the fact that it is possible to build an EFI by exploiting the unique feature of DH in extracting all the information content stored in hologram (phase and amplitude).

Paper Details

Date Published: 10 September 2005
PDF: 10 pages
Proc. SPIE 5908, Optical Information Systems III, 590803 (10 September 2005); doi: 10.1117/12.617101
Show Author Affiliations
P. Ferraro, Istituto Nazionale di Ottica Applicata, Sez. di Napoli (Italy)
S. Grilli, Istituto Nazionale di Ottica Applicata, Sez. di Napoli (Italy)
D. Alfieri, Istituto Nazionale di Ottica Applicata, Sez. di Napoli (Italy)
S. De Nicola, Istituto di Cibernetica del CNR (Italy)
A. Finizio, Istituto di Cibernetica del CNR (Italy)
G. Pierattini, Istituto di Cibernetica del CNR (Italy)
B. Javidi, Univ. of Connecticut (United States)
G. Coppola, Istituto di Microelettronica e Microsistemi del CNR (Italy)
V. Striano, Istituto di Microelettronica e Microsistemi del CNR (Italy)


Published in SPIE Proceedings Vol. 5908:
Optical Information Systems III
Bahram Javidi; Demetri Psaltis, Editor(s)

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