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Proceedings Paper

Optical trapping inside living organisms
Author(s): Poul Martin Hansen; Lene Broeng Oddershede
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Paper Abstract

We use optical tweezers to investigate processes happening inside ving cells. In a previous study, we trapped naturally occurring lipid granules inside living yeast cells, and used them to probe the viscoelastic properties of the cytoplasm. However, we prefer to use probes which can be specifically attached to various organelles within the living cells in order to optically quantify the forces acting on these organelles. Therefore, we have chosen to use nanometer sized gold beads as probes. These gold beads can be conjugated and attached chemically to the organelles of interest. Only Rayleigh metallic particles can be optically trapped and for these it is the case that the larger the beads, the larger the forces which can be exerted and thus measured using optical tweezers. The gold nanoparticles are injected into the cytoplasm using micropipettes. The very rigid cell wall of the S. pombe yeast cells poses a serious obstacle to this injection. In order to be able to punch a hole in the cell, first, the cells have to be turned into protoplasts, where only a lipid bilayer separates the cytoplasm from the surrounding media. We show how to perform micropipette delivery into the protoplasts and also how the protoplasts can be ablated using the trapping laserlight. Finally, we demonstrate that we can transform the protoplasts back to normal yeast cells.

Paper Details

Date Published: 26 August 2005
PDF: 9 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593003 (26 August 2005); doi: 10.1117/12.616879
Show Author Affiliations
Poul Martin Hansen, Niels Bohr Institute (Denmark)
Lene Broeng Oddershede, Niels Bohr Institute (Denmark)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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