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Proceedings Paper

Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers
Author(s): Ho-Chien Lin; Long Hsu
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Paper Abstract

Cell sorting is important in quantitative analysis for cellular and molecular biology study. For this purpose, we develop an opto-electro-mechanical sorting system which integrates a holographic optical tweezers (HOT) system to a microfluidics device. This system is designed to manipulate and sort various micro objects of different sizes by an optical pattern which is dynamic and programmable. In this work, we fabricate a microchannel with a main channel and two branches with polydimethylsiloxane (PDMS) which is a biocompatible elastomer material by utilizing semiconductor micro-fabrication techniques. By using a HOT system, we also generate two linear optical traps for the manipulation of micro particles. As a result, we have demonstrated that the larger particles are guided to the second branch by the two linear optical traps while the smaller particles remain unaffected and flow into the first branch of the microchannel.

Paper Details

Date Published: 26 August 2005
PDF: 8 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59301V (26 August 2005); doi: 10.1117/12.616743
Show Author Affiliations
Ho-Chien Lin, National Chiao Tung Univ. (Taiwan)
Long Hsu, National Chiao Tung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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