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Proceedings Paper

Growth of crystals in optical tweezers
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Paper Abstract

We report here on the use of optical tweezers in the growth and manipulation of protein and inorganic crystals. Sodium chloride and hen egg-white lysozyme crystals were grown in a batch process, and then seeds from the solution were introduced into the optical tweezers. The regular and controllable shape and the known optical birefringence in these structures allowed a detailed study of the orientation effects in the beam due to both polarization and gradient forces. Additionally, we determined that the laser tweezers could be used to suspend a crystal for three-dimensional growth under varying conditions. Studies included increasing the protein concentration, thermal cycling, and a diffusion-induced increase in precipitant concentration. Preliminary studies on the use of the tweezers to create a localized seed for growth from polyethylene oxide solutions are also reported.

Paper Details

Date Published: 26 August 2005
PDF: 9 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593014 (26 August 2005); doi: 10.1117/12.616739
Show Author Affiliations
Ursula Gibson, Dartmouth College (United States)
Wolfgang Singer, Univ. of Queensland (Australia)
Timo Nieminen, Univ. of Queensland (Australia)
Norman Heckenberg, Univ. of Queensland (Australia)
Halina Rubinsztein-Dunlop, Univ. of Queensland (Australia)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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