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Proceedings Paper

Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope
Author(s): Chia-Han Chan; Bo-Jui Chang; Ying-Jung Huang; Chia-Chieh Fan; Hwei-Ling Peng; Sien Chi; Long Hsu
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Paper Abstract

Swimming activity of flagella is a main factor of the motility of bacteria. Flagella expressed on the surface of bacterial species serve as a primary means of motility including swimming. We propose to use optical tweezers to analyze the swimming activity of bacteria. The sample bacteria in the work is Pseudomonas aeruginosa, and it is a gram-negative bacterium and often causes leading to burn wound infections, urinary-tract infections, and pneumonia. The single polar flagellum of P. aeruginosa has been demonstrated to be important virulence and colonization factor of this opportunistic pathogen. We demonstrate a gene to regulate the bacterial swimming activity in P. aeruginosa PAO1 by biological method. However, the change of flagellar morphology was not observed by electron microscopy analysis, suggesting that the gene regulates the flagellar rotation that could not be detected by biological method. PFM exhibits a spatial resolution of a few nanometers to detect the relative position of the probe at an acquisition rate over 1 MHz. By binding a probe such as a bead or a quantum dot on the flagella, we expect the rotation of the probe due to the flagella could be detected. It is expected that the study of the swimming activity of P. aeruginosa provide potent method for the pathogenic role of the flagella in P. aeruginosa.

Paper Details

Date Published: 18 August 2005
PDF: 8 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59300E (18 August 2005); doi: 10.1117/12.616704
Show Author Affiliations
Chia-Han Chan, National Chiao Tung Univ. (Taiwan)
Bo-Jui Chang, National Chiao Tung Univ. (Taiwan)
Ying-Jung Huang, National Chiao Tung Univ. (Taiwan)
Chia-Chieh Fan, National Chiao Tung Univ. (Taiwan)
Hwei-Ling Peng, National Chiao Tung Univ. (Taiwan)
Sien Chi, National Chiao Tung Univ. (Taiwan)
Long Hsu, National Chiao Tung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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