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Proceedings Paper

Measurement of point-spread function (PSF) for confocal fluorescence microscopy
Author(s): InCheon Song; HongKi Yoo; Jaebum Choo; Dae-Gab Gweon
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Paper Abstract

In this paper, we describe size-effect of fluorescent microsphere for measuring point-spread function (PSF) in confocal fluorescence microscopy. We present the numerical results for the practically available microsphere size range for measuring PSF, and demonstrate with experiment. Also, the effective PSF is restored with deconvolution technique within an acceptable error. Also the size-effect for measuring phase modulated PSF, which has two vicinal peaks, is described. The numerical and experimental result is also presented.

Paper Details

Date Published: 1 September 2005
PDF: 9 pages
Proc. SPIE 5878, Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II, 58781B (1 September 2005); doi: 10.1117/12.616701
Show Author Affiliations
InCheon Song, Korea Advanced Institute of Science and Technology (South Korea)
HongKi Yoo, Korea Advanced Institute of Science and Technology (South Korea)
Jaebum Choo, Hanyang Univ. (South Korea)
Dae-Gab Gweon, Korea Advanced Institute of Science and Technology (South Korea)


Published in SPIE Proceedings Vol. 5878:
Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II
Angela Duparre; Bhanwar Singh; Zu-Han Gu, Editor(s)

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