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Proceedings Paper

Dynamics of single DNA molecules under femtonewton forces
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Paper Abstract

Studying the thermal fluctuations of DNA molecules reveals not only a wealth of interesting equilibrium and nonequilibrium statistical mechanics, but is also of importance for understanding the dynamics of DNA in vivo. An instance of the latter is in the context of regulatory functions that require collaborative interactions of distant operator sites on the DNA molecule. These thermal fluctuations are extremely sensitive to mechanical constraints, such as supercoiling or mechanical tension in the DNA. The natural force scale fc on which these fluctuations are sensitive to tension is related to the persistence length lp by fc = kBT/lp = 80 fN, which is generally considered small for a crowded cellular environment. We are studying the dynamics of single DNA molecules under tension under equilibrium and non-equilibrium conditions using a modified scanning-line laser trap. This technique allows us to apply a constant force between 20 fN and 3 pN to a λ-DNA molecule while we study its behavior under two different conditions: 1) nonequilibrium studies in which we observe the relaxation trajectory of a highly extended DNA molecule as it returns to its equilibrium conformation against an applied optical force, and 2) equilibrium studies which measure fluctuations in the extension of the molecule about its mean with sub-millisecond time resolution as a function of its extension. In the nonequilibrium studies we find a marked deviation from predictions derived from the wormlike-chain (WLC) model for extended DNA molecules. In the equilibrium studies we compute the time-correlation functions of the fluctuations to determine their time constants, and model them with a simple bead-and-spring model. We observe a decrease of the fundamental time constant with increasing extension of the molecule. This suggests that the change in spring constant dominates changes in the intra-chain hydrodynamic coupling between segments as the Gaussian coil unravels into an extended conformation.

Paper Details

Date Published: 18 August 2005
PDF: 9 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593010 (18 August 2005); doi: 10.1117/12.615985
Show Author Affiliations
Rajalakshmi Nambiar, Univ. of Michigan (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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