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Proceedings Paper

Correlation of sperms’ swimming force to their swimming speed as assessed by optical tweezers
Author(s): Jaclyn Vinson; Elliot Botvinick; Barbara Durrant; Michael Berns
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Paper Abstract

The purpose of this study is to seek a correlation between the swimming forces of sperm, their swimming speed and the speed of progression score (SOP), which is given to them by the generally applied subjective 1 - 5 system. This study also examines the effects of length of exposure to the laser trap and laser power on sperm motility. Sperm with SOPs of 2-4 were trapped by a continuous wave 1064 nm single point gradient laser trap. To study trap duration effects, sperm were trapped for fifteen, ten and five seconds at 420mW laser power. To study laser power effects within the trap, powers of 420mW, 350mW, 300mW, and 250mW were applied for a constant duration (5 seconds). The correlations between sperm swimming force, swimming speed and speed of progression were examined at a trap duration and trapping power that were statistically shown to not significantly affect sperm motility. Swimming forces were measured by trapping sperm and subsequently decreasing laser power until the sperm were capable of escaping the trap (escape laser power is directly proportional to swimming force). Swimming speeds were calculated by custom built software, and speed of progression scores were assigned in a blind study by fertility experts examining offline video of the experiments. Sperm swimming force measurements by optical trapping may be a method to quantitatively measure sperm vitality that augments currently used methods.

Paper Details

Date Published: 26 August 2005
PDF: 10 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593026 (26 August 2005); doi: 10.1117/12.614505
Show Author Affiliations
Jaclyn Vinson, Univ. of California, San Diego (United States)
Elliot Botvinick, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)
Barbara Durrant, Beckman Ctr. for Conservation and Research for Endangered Species (United States)
Michael Berns, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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