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Proceedings Paper

Multispectral endoscopy and microscopy imaging system using a spectrally programmable light engine
Author(s): N. MacKinnon; Ulrich Stange; Pierre Mark Lane; Calum E. MacAulay
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Paper Abstract

We report a spectrally and temporally programmable light engine based on a spatial light modulator that can dynamically create any narrow or broadband spectral profile for hyperspectral, fluorescence, or principal component imaging. Most hyperspectral or multispectral imaging systems use wavelength selection devices such as acousto-optic tunable filters (AOTFs), tunable grating or prism-based monochromators, or filter wheels. While these devices can select wavelengths they cannot create arbitrary spectral profiles. This simple and economical system can be controlled at high speed (up to 5000 illumination profiles per second). Digitally controlled illumination is bit additive with image data providing high dynamic range imaging with monochrome or color imaging devices. This is especially advantageous for endoscopes employing small well CCD or CMOS sensors since the dynamic range now can extend beyond the limits of the sensor itself. In this report we show multispectral images of in vivo tissue and in vitro tissue samples using endoscopes, surgical microscopes and conventional microscopes.

Paper Details

Date Published: 23 March 2005
PDF: 6 pages
Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.613989
Show Author Affiliations
N. MacKinnon, British Columbia Cancer Research Ctr. (Canada)
Ulrich Stange, British Columbia Cancer Research Ctr. (Canada)
Pierre Mark Lane, British Columbia Cancer Research Ctr. (Canada)
Calum E. MacAulay, British Columbia Cancer Research Ctr. (Canada)


Published in SPIE Proceedings Vol. 5694:
Spectral Imaging: Instrumentation, Applications, and Analysis III
Gregory H. Bearman; Anita Mahadevan-Jansen; Richard M. Levenson, Editor(s)

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