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Proceedings Paper

Spectrally resolved fluorescence lifetime and FRET measurements
Author(s): Christoph U. Biskup; Laimonas Kelbauskas; Thomas Zimmer; Sascha Dietrich; Klaus Benndorf; Wolfgang Becker; Axel Bergmann; Nikolaj Klocker
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Paper Abstract

We present two different approaches that allow multi-wavelength fluorescence lifetime measurements in the time domain in conjunction with a laser scanning microscope and a pulsed excitation source. One technique is based on a streak camera system, the other technique is based on a time-correlated-single-photon-counting (TCSPC) approach. When applied to Forster resonance energy transfer (FRET) measurements, these setups are capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.

Paper Details

Date Published: 30 March 2005
PDF: 9 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.613006
Show Author Affiliations
Christoph U. Biskup, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Laimonas Kelbauskas, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Thomas Zimmer, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Sascha Dietrich, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Klaus Benndorf, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Axel Bergmann, Becker & Hickl GmbH (Germany)
Nikolaj Klocker, Albert-Ludwigs-Univ. Freiburg (Germany)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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