Share Email Print
cover

Proceedings Paper

Spectrally resolved fluorescence lifetime and FRET measurements
Author(s): Christoph U. Biskup; Laimonas Kelbauskas; Thomas Zimmer; Sascha Dietrich; Klaus Benndorf; Wolfgang Becker; Axel Bergmann; Nikolaj Klocker
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present two different approaches that allow multi-wavelength fluorescence lifetime measurements in the time domain in conjunction with a laser scanning microscope and a pulsed excitation source. One technique is based on a streak camera system, the other technique is based on a time-correlated-single-photon-counting (TCSPC) approach. When applied to Forster resonance energy transfer (FRET) measurements, these setups are capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.

Paper Details

Date Published: 30 March 2005
PDF: 9 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.613006
Show Author Affiliations
Christoph U. Biskup, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Laimonas Kelbauskas, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Thomas Zimmer, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Sascha Dietrich, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Klaus Benndorf, Becker & Hickl GmbH (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Axel Bergmann, Becker & Hickl GmbH (Germany)
Nikolaj Klocker, Albert-Ludwigs-Univ. Freiburg (Germany)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top