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Proceedings Paper

Effectiveness of photosensitive dye during uptake and redistribution
Author(s): Xiao-Yan He; Steven L. Jacques; Gary Gofstein
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Paper Abstract

The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

Paper Details

Date Published: 1 June 1992
PDF: 11 pages
Proc. SPIE 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, (1 June 1992); doi: 10.1117/12.60943
Show Author Affiliations
Xiao-Yan He, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Steven L. Jacques, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Gary Gofstein, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 1645:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy
Thomas J. Dougherty, Editor(s)

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