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Proceedings Paper

Toward a biophotonic MEMS cell sensor
Author(s): Michael A. Powers; Stephan T. Koev; Arne Schleunitz; Hyunmin Yi; Vildana Hodzic; William E. Bentley; Gregory F. Payne; Gary W. Rubloff; Reza Ghodssi
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Paper Abstract

We present a new platform for the optical analysis of biomolecules based upon the polysaccharide chitosan. The versatile, stable, and compatible nature of chitosan makes it an ideal material for integrating biological materials in microfabricated systems. Chitosan’s pH-responsive solubility allows electrochemical deposition, while its chemical reactivity enables facile coupling of proteins, oligonucleotides, and other biomolecules by covalent bonds. This work demonstrates the spatially selective assembly of a fluorescent molecule on chitosan and its applicability to microscale optical transducers. We define multimode waveguides and fluidic channels on a Pyrex wafer using a single layer of SU-8. Our implementation of sidewall patterning of transparent electrodes (indium tin oxide) on SU-8 structures is demonstrated and can be highly beneficial to fluorescent signal transduction. In this optical configuration, normally incident excitation light illuminates a chitosan surface on the vertical face of a collector waveguide intersected by a microfluidic channel. We demonstrate the collection of the optical signal in the integrated waveguide and analyze the signal by coupling the waveguide to a grating spectrometer.

Paper Details

Date Published: 29 June 2005
PDF: 8 pages
Proc. SPIE 5839, Bioengineered and Bioinspired Systems II, (29 June 2005); doi: 10.1117/12.608531
Show Author Affiliations
Michael A. Powers, Univ. of Maryland/College Park (United States)
Stephan T. Koev, Univ. of Maryland/College Park (United States)
Arne Schleunitz, Univ. of Maryland/College Park (United States)
Hyunmin Yi, Univ. of Maryland/College Park (United States)
Vildana Hodzic, Univ. of Maryland/College Park (United States)
William E. Bentley, Univ. of Maryland/College Park (United States)
Gregory F. Payne, Univ. of Maryland/College Park (United States)
Gary W. Rubloff, Univ. of Maryland/College Park (United States)
Reza Ghodssi, Univ. of Maryland/College Park (United States)

Published in SPIE Proceedings Vol. 5839:
Bioengineered and Bioinspired Systems II
Ricardo A. Carmona; Gustavo Linan-Cembrano, Editor(s)

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