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Proceedings Paper

The quantum dot nanoconjugate tool box
Author(s): I. D. Tomlinson; D. W. Wright; T. D. Giorgio; R. D. Blakely; S. J. Pennycook; D. Hercules; L. Bentzen; R. A. Smith; J. McBride; M. J. Vergne; S. Rosenthal
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Paper Abstract

The surface coating of quantum dots has been characterised using Z-stem. Quantum dots have been pegylated to increase stability in aqueous solution. The fluorescence intensity of the quantum dots was modulated pegylation. PEG was coupled using different ratios of EDC, PEG and NHS. Optimum coupling conditions were found to occur when 2000 equivalents of PEG were reacted with 1 equivalent of dot in the presence of 1500 equivalents of NHS and EDC. Angiotensin II was also conjugated to quantum dots and these conjugates were shown to be biologically active. Quantum dots have also been surface functionalised with other peptides such as NGR with subsequent demonstration of cell surface binding and can be characterized by flow cytometry.

Paper Details

Date Published: 28 April 2005
PDF: 11 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.606592
Show Author Affiliations
I. D. Tomlinson, Vanderbilt Univ. (United States)
D. W. Wright, Vanderbilt Univ. (United States)
T. D. Giorgio, Vanderbilt Univ. (United States)
R. D. Blakely, Vanderbilt Univ. School of Medicine (United States)
S. J. Pennycook, Oak Ridge National Lab. (United States)
D. Hercules, Vanderbilt Univ. (United States)
L. Bentzen, Vanderbilt Univ. (United States)
R. A. Smith, Vanderbilt Univ. (United States)
J. McBride, Vanderbilt Univ. (United States)
M. J. Vergne, Vanderbilt Univ. (United States)
S. Rosenthal, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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