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Proceedings Paper

Dark fraction and blinking of water-soluble quantum dots in solution
Author(s): Jie Yao; Daniel R. Larson; Warren R. Zipfel; Watt W. Webb
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Paper Abstract

Water-soluble quantum dots (qdots) have been introduced as bright fluorophores into life sciences research. Although various photophysical pathologies of qdots have been found, how their biological applications will be affected --- particularly in the native biological environment --- has not been evaluated. By fluorescence coincidence analysis and fluorescence cross-correlation spectroscopy, we studied the dark fraction of free-diffusing qdots in aqueous solution. We were able to detect individual qdots and found significant heterogeneity --- well-distinguished dark qdots and bright qdots. We estimated the bright fraction of Qdot525-Streptavidin to be about 55%. Blinking events were also noticed in fluorescence coincidence analysis, with “on/off” timescale from submilliseconds to tens of milliseconds.

Paper Details

Date Published: 28 April 2005
PDF: 4 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.606131
Show Author Affiliations
Jie Yao, Cornell Univ. (United States)
Daniel R. Larson, Cornell Univ. (United States)
Warren R. Zipfel, Cornell Univ. (United States)
Watt W. Webb, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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