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Proceedings Paper

Optical monitoring of proteins at solid interfaces
Author(s): G. Dunne; L. McDonnell; R. Miller; N. D. McMillan; B. O'Rourke; C. I. Mitchell
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Paper Abstract

The adsorption properties of polymers are of great importance for implant studies. A better understanding of these properties can lead to improved implant materials. In this study the surface energy of different polymers was derived from contact angle measurements taken using profile analysis tensiometry (PAT) of sessile drops of water. The contact angles were measured for advancing and receding water drops on polished polymer surfaces and also on polymer surfaces modified by adsorbing protein to the surface prior to analysis of the sessile drop. The protein used was bovine serum albumin (BSA) and the surfaces were poly-methylmethacrylate (PMMA), poly-ether-ether-ketone (PEEK) and stainless steel. The polymer surfaces were also studied using atomic force microscopy (AFM). Images of the surfaces were taken in different states: rough, smooth and with albumin adsorbed. As a method to identify the proteins on the surface easier, anti-albumin antibodies with 30nm nano gold particles attached were adsorbed to the albumin on the surfaces. Using nano gold particles made the imaging more straightforward and thus made identification of the protein on the surface easier. The results from this work show the differing hydrophobicities of polymer surfaces under different conditions and a new nanotechnological method of protein identification.

Paper Details

Date Published: 2 June 2005
PDF: 6 pages
Proc. SPIE 5824, Opto-Ireland 2005: Nanotechnology and Nanophotonics, (2 June 2005); doi: 10.1117/12.605430
Show Author Affiliations
G. Dunne, Institute of Technology Carlow (Ireland)
L. McDonnell, Cork Institute of Technology (Ireland)
R. Miller, Max-Planck Institute for Colloids and Interfaces (Germany)
N. D. McMillan, Institute of Technology Carlow (Ireland)
B. O'Rourke, Institute of Technology Carlow (Ireland)
C. I. Mitchell, Institute of Technology Carlow (Ireland)

Published in SPIE Proceedings Vol. 5824:
Opto-Ireland 2005: Nanotechnology and Nanophotonics
David Kennedy; Werner J. Blau; John Colreavy, Editor(s)

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