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Proceedings Paper

Three dimensional confocal microscopy of fluorescent microspheres: imaging and size determination
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Paper Abstract

In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melamine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.

Paper Details

Date Published: 8 June 2005
PDF: 6 pages
Proc. SPIE 5827, Opto-Ireland 2005: Photonic Engineering, (8 June 2005); doi: 10.1117/12.604380
Show Author Affiliations
Y. P. Rakovich D.V.M., Trinity College Dublin (Ireland)
L. Yang, Freie Univ. Berlin (Germany)
C. M. Taylor, Trinity College Dublin (Ireland)
L. A. Dunbar, Institut de Photonique et d'Electronique Quantique, EPFL (Switzerland)
Aaron Mac Raighne, Trinity College Dublin (Ireland)
J. F. Donegan, Trinity College Dublin (Ireland)
Eithne M. McCabe, Trinity College Dublin (Ireland)


Published in SPIE Proceedings Vol. 5827:
Opto-Ireland 2005: Photonic Engineering
Thomas J. Glynn; John T. Sheridan; Brian W. Bowe; Ronan F. O'Dowd; Gerard M. O'Connor; Aidan J.H. Flanagan; Gerard D. O'Sullivan; Gerald Byrne; Jonathan Magee, Editor(s)

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