Share Email Print
cover

Proceedings Paper

High-throughput screening of metal chelating compounds
Author(s): Edel M. Minogue; George J. Havrilla; Tammy P. Taylor; Anthony K. Burrell; Benjamin P. Warner
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

An effective high throughput screening technique is described for the rapid analysis of solid-supported libraries to identify selective chelating agents for metals, focusing on those possibly present in radioactive dispersal devices. Micro X-ray Fluorescence (MXRF) is the method of choice. MXRF is a rapid and sensitive technique which allows the measurement of metal-chelator complexes without significant sample preparation. For this study we have employed commercial peptide libraries and have examined factors such as side-chain charge, oligopeptide length, hydrophobicity, and aromaticity in relation to metal binding. MXRF provides a rapid and quantitative means for screening chelator-ion binding. Initial experiments carried out successfully identified sequences that are selective for Co under certain binding conditions containing possible interferences (e.g. Ca, Fe, Al, Cs, Ir), which could be encountered in our application.

Paper Details

Date Published: 29 March 2005
PDF: 5 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.601911
Show Author Affiliations
Edel M. Minogue, Los Alamos National Lab. (United States)
George J. Havrilla, Los Alamos National Lab. (United States)
Tammy P. Taylor, Los Alamos National Lab. (United States)
Anthony K. Burrell, Los Alamos National Lab. (United States)
Benjamin P. Warner, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

© SPIE. Terms of Use
Back to Top