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Proceedings Paper

3D perfusion mapping in the intact mouse heart after myocardial infarction using myocardial contrast echocardiography
Author(s): Yinbo Li; Zequan Yang; Brent A. French; John A. Hossack
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Paper Abstract

An intact mouse model of surgically-induced myocardial infarction (MI) caused by permanent occlusion of the Left Anterior Descending (LAD) coronary artery was studied. Normal mice with no occlusion were also studied as controls. For each mouse, contrast enhanced ultrasound images of the heart were acquired in parallel cross-sections perpendicular to the sternum at millimeter increments. For accurate 3D reconstruction, ECG gating and a tri-axial adjustable micromanipulator were used for temporal and spatial registration. Ultrasound images at steady-state of blood refilling were color-coded in each slice to show relative perfusion. Myocardial perfusion defects and necrosis were also examined postmortem by staining with Phthalo blue and TTC red dyes. Good correlation (R>0.93) in perfused area size was observed between in vivo measurements and histological staining. A 3D multi-slice model and a 3D rendering of perfusion distribution were created and showed a promising match with postmortem results, lending further credence to its use as a more comprehensive and more reliable tool for in vivo assessment of myocardial perfusion than 2D tomographic analysis.

Paper Details

Date Published: 12 April 2005
PDF: 8 pages
Proc. SPIE 5750, Medical Imaging 2005: Ultrasonic Imaging and Signal Processing, (12 April 2005); doi: 10.1117/12.601623
Show Author Affiliations
Yinbo Li, Univ. of Virginia (United States)
Zequan Yang, Univ. of Virginia (United States)
Brent A. French, Univ. of Virginia (United States)
John A. Hossack, Univ. of Virginia (United States)

Published in SPIE Proceedings Vol. 5750:
Medical Imaging 2005: Ultrasonic Imaging and Signal Processing
William F. Walker; Stanislav Y. Emelianov, Editor(s)

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