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Proceedings Paper

Toward the in vivo study of captopril-conjugated quantum dots
Author(s): Noriyoshi Manabe; Akiyoshi Hoshino; Yi-qiang Liang; Tomomasa Goto; Norihiro Kato; Kenji Yamamoto
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Paper Abstract

Photo-luminescent semiconductor quantum dots are nanometer-size probes that have the potential to be applied to the fields of the bio-imaging and the study of the cell mobility inside the body. At the same time, on the other hand, quantum dots are expected to carry some kind of molecules to the local organ inside of the animal body, which leads to the expectation that they can be used as a medicine-carrier. For this purpose, we conjugate (2S)-1-[(2s)-2-Methyl-3-sulfanylpropionyl]pyrrolidine-2-carboxylic acid (cap) with the quantum dot. Cap has the effect as an anti-hypertension drug, which inhibits angiotensin 1 converting enzyme. We conjugated the quantum dot with cap by the exchange reaction avoiding the regions which holds medicinal effect. Quantum dot conjugated with cap (QD-cap) were 3-times brighter than thioglycerol-coated quantum dots (QD-OH). The particle size of cap was 1.1nm and that of QD-cap was 12nm. QD-cap was permeated into the HeLa cells, while QD-MUA were taken into the HeLa cells by endocytosis. In addition, no apoptosis was detected against the cells that permeated QD-cap, because there was no damage to DNA. These results indicated that QD-conjugated medicines (QD-medicine) could be safe in the experiment on the level of the cell. More over, when QD-cap was intravenously injected into Stroke-prone Spontaneously Hypertensive Rats (SHRSP), they reduced blood pressure at systole. Therefore, the anti-hypertension effect of cap remained after conjugated with the quantum dot. These results suggested that QD-medicine were effective on the animal level.

Paper Details

Date Published: 28 April 2005
PDF: 11 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.601615
Show Author Affiliations
Noriyoshi Manabe, Research Institute of the International Medical Ctr. of Japan (Japan)
Akiyoshi Hoshino, Research Institute of the International Medical Ctr. of Japan (Japan)
Yi-qiang Liang, Research Institute of the International Medical Ctr. of Japan (Japan)
Tomomasa Goto, Research Institute of the International Medical Ctr. of Japan (Japan)
Norihiro Kato, Research Institute of the International Medical Ctr. of Japan (Japan)
Kenji Yamamoto, Research Institute of the International Medical Ctr. of Japan (Japan)


Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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