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Proceedings Paper

Fluorescent labeling of cells and biomolecules with nanocrystal quantum dots
Author(s): Akiyoshi Hoshino; Kouki Fujioka; Masakazu Suga; Yu F. Sasaki; Toshihiro Ohta; Masato Yasuhara; Taeko Dohi; Kazuo Suzuki; Kenji Yamamoto
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Paper Abstract

Fluorescent nanoparticles, such as nanocrystal quantum dots (QDs), novel nanometer-size probes and have the potential to be used as easy imaging tool for molecular biology and bioimaging including medical applications, since some nanocrystals emit higher and far longer fluorescence than conventional organic probes. QDs are now becoming widely used in biotechnology and medical applications. QDs have several advantages over organic fluorophores with regard to high luminescence, stability against photobleaching, and a range of fluorescence wavelengths from blue to infrared depending on the particle size. In this review, we reported labeling of some kinds of immune cells and biomolecules with several QDs coated with hydrophilic carboxyl/amine groups, and reported that we could image the circulation of mouse lymphocytes in vivo by QDs. In addition, we also reported here about the cytotoxicity of these nanocrystals.

Paper Details

Date Published: 28 April 2005
PDF: 9 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.601614
Show Author Affiliations
Akiyoshi Hoshino, Research Institute of the International Medical Ctr. of Japan (Japan)
Tokyo Medical and Dental Univ. (Japan)
Kouki Fujioka, Research Institute of the International Medical Ctr. of Japan (Japan)
Masakazu Suga, Research Institute of the International Medical Ctr. of Japan (Japan)
Yu F. Sasaki, Hachinohe National College of Technology (Japan)
Toshihiro Ohta, Tokyo Univ. of Pharmacy and Life Science (Japan)
Masato Yasuhara, Tokyo Medical and Dental Univ. (Japan)
Taeko Dohi, Research Institute of the International Medical Ctr. of Japan (Japan)
Kazuo Suzuki, National Institute of Infectious Diseases (Japan)
Kenji Yamamoto, Research Institute of the International Medical Ctr. of Japan (Japan)
Tokyo Medical and Dental Univ. (Japan)


Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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