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Proceedings Paper

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis
Author(s): Greg J. McGraw; Rafael V. Davalos; John D. Brazzle; John T. Hachman; Marion C. Hunter; Jeffery M. Chames; Gregory J. Fiechtner; Eric B. Cummings; Yolanda Fintschenko; Blake A. Simmons
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Paper Abstract

We have successfully demonstrated selective trapping, concentration, and release of various biological organisms and inert beads by insulator-based dielectrophoresis within a polymeric microfluidic device. The microfluidic channels and internal features, in this case arrays of insulating posts, were initially created through standard wet-etch techniques in glass. This glass chip was then transformed into a nickel stamp through the process of electroplating. The resultant nickel stamp was then used as the replication tool to produce the polymeric devices through injection molding. The polymeric devices were made of Zeonor 1060R, a polyolefin copolymer resin selected for its superior chemical resistance and optical properties. These devices were then optically aligned with another polymeric substrate that had been machined to form fluidic vias. These two polymeric substrates were then bonded together through thermal diffusion bonding. The sealed devices were utilized to selectively separate and concentrate a variety of biological pathogen simulants and organisms. These organisms include bacteria and spores that were selectively concentrated and released by simply applying D.C. voltages across the plastic replicates via platinum electrodes in inlet and outlet reservoirs. The dielectrophoretic response of the organisms is observed to be a function of the applied electric field and post size, geometry and spacing. Cells were selectively trapped against a background of labeled polystyrene beads and spores to demonstrate that samples of interest can be separated from a diverse background. We have implemented a methodology to determine the concentration factors obtained in these devices.

Paper Details

Date Published: 22 January 2005
PDF: 10 pages
Proc. SPIE 5715, Micromachining and Microfabrication Process Technology X, (22 January 2005); doi: 10.1117/12.597711
Show Author Affiliations
Greg J. McGraw, Sandia National Labs. (United States)
Rafael V. Davalos, Sandia National Labs. (United States)
John D. Brazzle, Sandia National Labs. (United States)
John T. Hachman, Sandia National Labs. (United States)
Marion C. Hunter, Sandia National Labs. (United States)
Jeffery M. Chames, Sandia National Labs. (United States)
Gregory J. Fiechtner, Sandia National Labs. (United States)
Eric B. Cummings, Sandia National Labs. (United States)
Yolanda Fintschenko, Sandia National Labs. (United States)
Blake A. Simmons, Sandia National Labs. (United States)

Published in SPIE Proceedings Vol. 5715:
Micromachining and Microfabrication Process Technology X
Mary-Ann Maher; Harold D. Stewart, Editor(s)

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